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BluePippin DNA size selection system. The Sage Science BluePip

DNA size-selection was performed using Blue Pippin 2% dye free cassette with internal standard marker V1 (Sage Science). The size-selected product was equalized using the Ion Equalizer kit (LifeTechnologies, ThermoFisher Scientific) following manufacturer's instructions. All purifications used Agencourt AMPure XP Reagent (Beckman Coulter).Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole-genome sequences of two B. cereus strains isolated from ...Size-selection was performed using the Blue Pippin electrophoresis platform (Sage Science), in order to select fragments of 215 bp of length as in [ 70 ], in order to be able to integrate our data ...Blue Pippin. The platform features the ability for targeted size selection of DNA fragments (90bp-50kb) with minimal effort for the purpose of Next-Generation Sequencing. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross ...BluePippin Operations Manual 460013 Rev E 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.Posted on August 3, 2023 by Alex. Range+T Size Selection Sage's DNA size selection technology has been used to help improve PacBio sequencing, particularly for mammalian and complex genome work since the introduction of the BluePippin (2012). Over the last few years, PacBio®'s Hi-Fi circular consensus sequencing … more ».The desired product was confirmed by running agarose gel (0.8%) electrophoresis and purified by using Blue pippin (Sage Science) (Supplementary Fig. 1b). Single-molecule distance measurement using ...The Blue Pippin is a size selection tool (90bp – 50kb). With pulsed-field electrophoresis it can resolve and collect high molecular weight DNA. Applications include. miRNA isolation; Mate …This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...Clean-lined Pippin comes with a tailored, zip-on, zip-off cover that gives the chair its sophisticated personality. Pippin comes in NaughtOne's wide selection of fabrics and finishes, and it's also available with or without the handle and castors. While this lounge chair is built for longevity, it also considers the circular economy.The Pippin instrument software interface is accessed by pressing The Tabs on the main screen. If an instrument in connected to the group, the instrument's serial number will appear in the Tab heading. ... The protocol will be highlighted in blue. The associated cassette definition will automatically highlight. Master run protocols listThe blue pippin is a machine that is used to size-select DNA fragments from 100bp-50kbp.Target sizes or rangesof sizes are entered in software, and fractions are collected in buffer. Inquire Blue Pippin Standard DNA size selection cassette 3% agarosethe Pippin with the cover closed. Humidity from the cassette may cause damage to electrodes. Approximate Run Times for "Tight" Range Targets (hr:min) Run 1. Close the lid, go to the Main Tab, and make sure the proper protocol is loaded in the "Protocol Name" field. 2. Press "START". The run will automatically stop whenSage Science develops products for the life sciences research markets. Our mission is to provide new systems to streamline and improve sample preparation workflows for DNA and protein analysis. In 2010-12, the company commercialized the Pippin Prep and BluePippin preparative electrophoresis systems for targeted size selection of biomolecules. The Pippin platform has since established itself as ...News and Events 17-04 Reportage NRC handelsblad: 'Is het een schimmel, is het een virus?Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center. 22-12 PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and …The Blue Pippin also does your size selection, so you don't burn out pores sequencing lots of little stuff. This should help yield too. Then it's all down to the library prep, and @gringer's answer has some good tips on that. Share. Improve this answer. FollowBackground Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop …Blue Lakes. On December 23, Sgt. Walsh got a call from the Mini Golden Inn, the Inn said they had some of Noah's items in lost and found. The items were some clothing, chargers for a phone and laptop and a gun lock. However, Noah's phone, computer and gun were missing, so it's believed that Noah was armed when he disappeared into the ...Using Blue Pippin size selection, we have achieved yields of >500 M passed filter bases from individual SMRTcells. Below are the sequencing and assembly results of four genomes sequenced from long-insert, Blue Pippin size-selected libraries. Using only PacBio long subread data, we were able to assemble complete microbial genomes for three of ..."Old Tom Bombadil is a merry fellow! Bright Blue his jacket is, and his boots are yellow!" —Tom Bombadil Tom Bombadil was a mysterious being that lived for much of the history of the world, being known in the Third Age to dwell in the valley of the Withywindle in the depths of the Old Forest, east of Buckland, and close to the dangerous Barrow-downs. His domain was of modest size, but he ...N. crassa OR74A P4C2 SAMN02724975 BashingBeads, Zymo Research kit 6 none Blue Pippin (4kb) N. crassa T1 P4C2 SAMN02724976 SDS, proteinase K, phenol-chloroform, RNAase, isopropanol 15 none Blue ...The DNA was sheared to approximately 20kb, size separated using a Blue Pippin using the PAC-30 KB cassette (Sage Science). Libraries were prepared for PacBio sequencing using the large insert library protocol and Illumina sequencing using the TruSeq PCR-free kit per manufacturer recommendations. Long read sequencing was conducted on the Pacific ...Pippin: What about breakfast? Merry: You've already had it. Pippin: We've had one, yes. What about second breakfast? Merry: Don't think he knows about second breakfast, Pip. Pippin: What about elevenses? Luncheon? Afternoon tea? Dinner? Supper? He knows about them, doesn't he? Merry: I wouldn't count on it.Blue Pippin Specs: Electrophoresis voltage: 25V, 100V or 150V constant, 100V pulsed field: Optical detection: 470 nm excitation, 525 nm emission: Power requirements: 100 …PULSE1: Pippin Pulse Starter Bundle1, with Gel Box, Notebook, and KBB Buffer: $4,950.00The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a ‘living’ colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ...The BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ...Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.Welcome to Deep Seq. The sequencing facility offers research scientists access to state of the art high-throughput sequencing technology. Our experienced team of dedicated experimental scientists and bioinformaticians will work with you from the conception of your sequencing experiment to the final analysis of your data.Professional Freediver. Spouses. Audrey Mestre 1999-2002, Juscelina Cacau Melo 2012. Francisco Rodriguez, better known as Francisco 'Pipin' Ferreras (born January 18, 1962) is a Cuban free-diver known for his achievements in deep free diving and his relationship with his wife, Audrey Mestre .19 thg 6, 2013 ... Working with pacbio team also used blue pippin for size selection got the same result. It will be nice blue pippin give us better yield,it eats ...In the first of the six runs, involving 13 samples, we used a Blue Pippin instrument to size-select DNA amplicons before sequencing. This was done to separate microbiome from host mitochondrial 16S rRNA gene sequences. This method proved untenable due to Blue Pippin performance inconsistencies and we did not apply physical size-selection to the ...The gene structure of Pfpα is shown with exons in blue boxes and introns with a black line. ... and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries ...Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 - 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 - 10 kb Marker D1 CBC7601The pooled libraries were size selected for fragments between 300–500 bp on a 2% agarose gel using the Blue Pippin DNA Size Selection System (Sage Science, Beverly, MA) according to manufacturer protocol. Size-selected libraries were again checked for appropriate fragment length distribution and DNA quality using the Agilent 2100 Bioanalyzer ...Genomics Facility Depending on the results from the DNA sizing with the Fragment Analyzer, you may want to remove unwanted products from your library preparation. The Blue Pippin is used to select DNA of certain sizes and effectively remove unwanted fragment sizes.What is Pippin's Gender? Pippin is a non-binary character who uses they/them pronouns. It has always been our vision for Paleo Pines to have a diverse, inclusive community with characters that may reflect any player's identity or orientation. Will Lucky be the only Parasaur in the game? There will be others. :heart_eyes: Dev QuestionsWelcome to our Pippin Studio Collection! Pippin is a line of playful and original hand painted canvases designed in British Columbia, Canada and distributed from Pippin U.S. in Washington State. Pippin prides themselves on their original hand painted needlepoint canvas designs. You will fall in love with their lavish floral and festive patterns, pick out the perfect piece from our Stitching ...The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ...selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.13 thg 8, 2022 ... The Blue Tiger. oil on canvas • 40.64 x 71.12 cm. Horace Pippin - February 22, 1888 - July 6, 1946 Horace Pippin ...PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E.For Nanopore sequencing, large fragments of genomic DNA were obtained using the Blue Pippin automatic nucleic acid fragment recovery system (Sage Science, MA), and barcodes were added using the Nanopore EXP-NBD104 kit.Getting around town can be a hassle, especially if you don’t have your own car. But with Blue Van Shuttle Service, you can get to where you need to go quickly and easily. Here are some of the reasons why Blue Van Shuttle Service is the best...PacBio 10-20kb (Blue Pippin kit) 21ug 250ng/ul PacBio 30-40kb (Blue Pippin kit) 27ug 250ng/ul 10x Genomics -Chromium (BluePippin > 40kb size selection) 5ug 120ng/ul 10x Genomics - Chromium (no size selection)**** 400ng 15ng/ul * Recommended amounts for submission represent DNA quantities required for one library prep and additional QC.Abstract. The PacBio ® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%. These accurate long ...It's important to carefully inspect gel cassettes prior to use on the Pippin Prep or BluePippin. This instructional video from Sage Science shows how to quic...The BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ...For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...A collaboration between writer-actor-producer Sharon Diane King and writer-director-producer Curt Steindler (a partnership 21 years strong!), Blue Pippin Productions puts forth cinema that is engaged with social issues, often from a satirical, darkly comic perspective. The name honors two former muses of the filmmakers: the green iguanas Pippin and Blue.The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a ‘living’ colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ...Size selection with Blue-Pippin system (Sage Science) to remove fragments less than 15 Kb was done on purified sample with 0.45X AMPure PB beads (Pacific Biosciences). The size and concentration ...Cells were then counted using trypan blue staining. In total, 6,000 cells per well of a 96-well ultra-low attachment plate (Corning) were seeded. Once the plate was seeded, it was spun down at 234 ...The Blue Pippin also does your size selection, so you don't burn out pores sequencing lots of little stuff. This should help yield too. Then it's all down to the library prep, and @gringer's answer has some good tips on that. Contact for: PacBio, Blue Pippin, Bioruptor [email protected]. Siranoosh Ashtari. Contact for: NovaSeq, NextSeq & MiSeq sequencing, microRNA library preps, and Covaris [email protected]. Emily Kumimoto. Contact for: Illumina sequencing library preparations [email protected]. Ruta Sahasrabudhe, PhDBlue Pippin Size-Selection System. The first step of the protocol was cDNA synthesis with adapters. and PCR cycle optimization followed by large-scale PCR amplification. PCR products were then size.In total, 48 unique barcode adapters were used in initial ligation, and sub-pools of these 48 barcodes were size selected using a 1.5% agarose gel cassette and target size selection of “narrow 450 bp” on a Blue Pippin electrophoresis unit …For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...Sage Science size selecting with bluepippin Size Selecting With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.98 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the...Jan 5, 2012 · 98 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the... DNA fragments were amplified by PCR twice and size selected (300-450 bp) using a Blue Pippin. The final library was sequenced using a single-end 100 bp protocol within a lane of an Illumina Hiseq-4000 sequencer at the University of Texas at Austin Genome Sequencing and Analysis Facility (GSAF).Newtown pippin apples 2. Newtown Pippin apple. Newtown Pippin is a popular, all-American green apple from New York City. This apple has a smooth peel, often with a bit of gold russeting on it. The peel is a true green when freshly picked, but matures to a yellow as the apple becomes sweeter in storage.After purification of the second PCR product, fragments of a size suitable for sequencing (300–800 bp) are selected using a DNA size selection system (for example, Blue Pippin DNA Size Selection System; Sage Science, USA). 3. The DNA library of size-selected fragments is purified again with AMPureXP Beads.selection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq).Nick Pippin (16) QB. ... Official Home of Millikin Big Blue Athletics. Header logo. Menu. Sports. Men's ...Blue Apron Coupon. Teachers take $160 on their Blue Apron order. Since 1957, GQ has inspired men to look sharper and live smarter with its unparalleled coverage of style, culture, and beyond. From ...Pipkin's Market, Montgomery, Ohio. 4,794 likes · 10 talking about this · 882 were here. Your neighborhood Farmer's Market, only better! Fresh Produce & Curated Garden CenterPipis first appear as oval-shaped blue objects, resembling a balloon or an egg. When they pop, dozens of tiny Spamton faces fly out in every direction. Starting from version v1.07 there is a 1/100 chance of a "Ms. Pipis" replacing a regular Pipis, which sports a simple pink bow, but is otherwise identical to Pipis. Personality []May 2, 2019 · Good quality library: A good quality library will result in most of the pores being in the “Sequencing” state (neon green), and very few in “Pore” (green), “Recovering” (dark blue), or “Inactive” (light blue). A library that results in a Duty Time graph like the example below is likely to give a good sequencing throughput. Pippin. Sage Science 為專業的片段分離系統廠商,一直以來 Pippin Prep (100bp - 1.5kb)表現出卓越的分離結果,之後的Blue Pippin (100bp - 50kb) 更夾帶著Pulsed-field技術的高分辨率及回收率,令Sage Science 至今仍站穩全世界市占率最高的片段分離系統供應商,以及Blue Pippin ...Due to the limited input DNA amount of 1 μg, the library was not additionally size-selected by, for example, Blue Pippin or SAGE Science. Sequencing-by-synthesis was performed on a HiSeq 3000 with 2 × 150 bp paired-end reads at the Max Planck Genome Centre Cologne, Germany.Unsupported-Protocol-Bacterial-Iso_Seq-Clontech-SMARTer-PCR-cDNA-Synthesis-Kit-BluePippin-Size-SelectionThe desired product was confirmed by running agarose gel (0.8%) electrophoresis and purified by using Blue pippin (Sage Science) (Supplementary Fig. 1b). Single-molecule distance measurement using ...Built to make bath-time fun and enjoyable, this fold-away design makes storage easy without compromising a full-size tub for your little one.A PacBio Sequencing library was constructed using the SMRTbell TM Template Prep Kit 1.0 and by following the PacBio 20 kb library protocol; the library molecules, after adapter ligation and damage repair, were size selected for 15 kb and larger using Blue Pippin instrument (Sage Sciences) by following the manufacturer's instructions. The ...lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed usingselection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq).Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was used for the size selection of different sizes of cDNA libraries (1–2, 2–3, and > 3 kb). According to the instructions, three SMRT libraries were constructed using SMRTbell template prep kit 1.0 (Pacific Biosciences) and were sequenced through seven SMRT …For this reason, careful fragment size selection on the Blue Pippin during sample preparation can pay huge dividends during downstream data analysis. This instrument is also critical for the selection of high molecular weight DNA for 10X Whole Genome and PacBio long-read sequencing applications. library was size-selected using a Blue Pippin (Sage Science), retaining only those fragments 300 bp ± 50 bp in length. This size-selection range was chosen by performing an in silico diges-tion of the published prairie rattlesnake, C. viridis viridis, genome [43], with the cut-site sequences of PstI (CTGCA^G) and MspI (C^CGG) using the program ...Illumina flow cell (P5 shown in light blue, P7 shown in red), Illumina TruSeq HT (currently known as TruSeq CD) index sequences (Index 2 [i5] shown in orange, Index 1 [i7] shown in yellow), and sequences bound by the Read Primer 1 or Read Primer 2 sequencing primers (shown in purple and green, respectively).Check out our blue pippin fabric selection for the very best in unique or custom, handmade pieces from our fabric shops.Venues really don't come much more picturesque than PIPPIN HILL FARM in the heart of Virginia's wine country. It was an obvious choice for Spencer & Daniel ...No one really knows how tall Pepin the Short really was, but it is assumed he was actually quite tall. The Pippin family was actually tall, and the correct title of Pippin the Short was probably misinterpreted.Technical note ALTERNATIVE SIZE SELECTION METHODS FOR SMRTBELL ® PREP KIT 3.0 Introduction In Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0, size-selection beads are used to remove short DNA fragments and enrich for the long fragments that are most valuable for …Page 6 P/N 100-286-000-07 STEP Purify DNA Notes 1 Add 0.45X volume of AMPure PB beads to the End-Repair reaction. (For detailed instructions on AMPure PB bead purification, see the Concentrate DNA section.) 2 Mix the bead/DNA solution thoroughly. 3 Quickly spin down the tube (for 1 second) to collect the beads. Do not pellet beads.Pippin Pulse™, and the Agilent TapeStation for HMW DNA QC. To maximize Linked-Read application performance, gDNA >50 kb is acceptable, whereas gDNA >80 kb is ideal. If QC show a significant amount of gDNA <50 kb, the sample may be rescued by performing a size selection using the Sage Science Blue Pippin or PippinHT instruments.The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing platforms. Features : Saves time and effort compared to manual gel purification; Sample-to-sample reproducibility streamlines researchGeographic variation in West African Agama picticauda: insights from genetics, morphology and ecology Sneha Krishnana, Caleb Ofori-Boatengb, Matthew K Fujitac and Adam D Leachéd aColorado State University, College of Veterinary Medicine and Biomedical Sciences 1350 Center Ave, Fort Collins, CO 80521, Fort Collins, CO, USA 80526, 4257858934; bKwame Nkrumah University of Science andPippin Prep DNA Size Selection System Selects 100 bp – 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse ... ducted using the Blue Pippin automatic nucleic acid fragment recovery system. Then, barcode was added by a PCR-free method using the EXP-NBD104 kit (Oxford Nanopore Technologies Company). Afterward, the size of fragments was detected by the AATI auto-matic capillary electrophoresis instrument, and the samples were isomolar mixed. Finally, theIn our Iso-Seq analysis, we detected a total of 27,860 transcripts, of which 11,342 (40.7%) are novel. Furthermore, 7,065 (25.4%) were identified as full-length, that is, the starting exon of an ...Fragments smaller than 20 kbp were removed using the Blue Pippin Size selection system (Sage Science, Beverly, MA, USA) and the constructed libraries were validated using Agilent 2100 Bioanalyzer .... Pipkin's Market, Montgomery, Ohio. 4,794The entire elution was size selected on a 3% agarose cassette usi Find many great new & used options and get the best deals for Sage Science BluePippin BR02552 50bp-30kb DNA Size Selection System Blue Pippin at the best online prices at eBay! Free shipping for many products!The High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to our BluePippin "High-Pass" DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing. ... (100% up from standard Blue Pippin cassettes). We've also increased the size of ... The library was then reduced to fragments Size selection was performed on Sage blue pippin prep using 0.75% agarose cassette and S1 marker using a cutoff of 6 kb. The final SMRT bell was recovered as before and quantified by Qubit assay and fragment analyzer. SMRTbell library was annealed to sequencing primer at values predetermined by the Binding Calculator (Pac Bio) and a complex ...16K views 11 years ago. This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the … Blue Pippin回收的片段范围:50bp-50kp,样品通量:1-5个样品,回收效率:50-80%。...

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